昆虫类钙粘蛋白与Bt Cry1A蛋白之间的相互作用
The interactions between insect cadherin-like proteins and Bt Cry1A protein
韩岚岚，赵奎军，张 杰
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作者单位：东北农业大学农学院
中文关键词：类钙粘蛋白，Bt Cry蛋白，苏云金芽孢杆菌，受体
英文关键词：cadherin-like protein, Bt Cry protein, Bacillus thuringiensis, receptor
中文摘要：类钙粘蛋白(cadherin-like protein)位于昆虫中肠刷状缘膜囊泡(brush border membrane vesicles, BBMV)上，是苏云金芽孢杆菌(Bacillus thuringiensis，Bt)产生的杀虫晶体蛋白(Bt Cry蛋白)的主要受体之一。它能够与Bt Cry蛋白结合，引起细胞膜的渗透性发生改变，促进Bt Cry蛋白对敏感昆虫的毒杀作用。类钙粘蛋白基因的突变还能导致敏感昆虫对Bt Cry蛋白产生抗性。因此，研究昆虫类钙粘蛋白与Bt Cry蛋白之间的相互作用，将有助于揭示Bt Cry蛋白杀虫作用机理。文章对昆虫类钙粘蛋白种类、结构特征、在昆虫体内的分布、及其与Bt Cry蛋白之间的相互作用等方面的研究现状进行详细论述。
英文摘要：The cadherin-like proteins, major receptors of Bacillus thuringiensis insecticidal crystal protein (Bt Cry protein), are usually anchored to the membrane in the brush border membrane vesicles (BBMV) of insect midgut. The cadherin-like proteins may band with Cry1A protein so that cause change of ionic permeability and increase toxicity to susceptible insects. The mutation of insect cadherin-like proteins may result in resistance to Bt Cry protein. Therefore, the study on interaction between cadherin-like proteins and Bt Cry protein would be helpful for understanding insecticidal mechanism of Cry protein. In this paper, cadherin-like protein type, characteristics of protein structure, distribution inside of insect, and the interaction between insect cadherin-like proteins and Bt Cry1A protein were reviewed.