稻纵卷叶螟羧酸酯酶基因的鉴定与表达谱分析
Identification and expression profiles of Cnaphalocrocis medinalis carboxylesterase genes
刘 苏** 冯明峰 何梦竹
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DOI：10.7679/j.issn.2095-1353.2015.077
作者单位：安徽农业大学植物保护学院，合肥 230036
中文关键词：稻纵卷叶螟，羧酸酯酶，系统进化分析，表达谱
英文关键词：Cnaphalocrocis medinalis, carboxylesterase, phylogenetic analysis, expression profiles
中文摘要： 【目的】 鉴定稻纵卷叶螟Cnaphalocrocis medinalis的羧酸酯酶基因，并检测这些基因在成虫不同组织中的表达模式。【方法】 从稻纵卷叶螟转录组中搜索羧酸酯酶基因，使用生物信息学软件对所获得的基因序列进行分析，使用荧光定量PCR检测这些基因在各组织中的相对表达水平。【结果】 获得了15个羧酸酯酶基因，分别命名为CmCarE1~CmCarE15。其中CmCarE12缺少3′区域，其余14个序列均含有完整的开放阅读框。除CmCarE11外，其余14个基因编码的蛋白均具有羧酸酯酶的典型特征，如保守的五肽结构域，催化三联体和氧阴离子穴等。系统进化分析显示15个CmCarEs被聚在不同的进化支内，CmCarE13、CmCarE14和CmCarE15聚在“胞内催化类”进化支，其余12个CmCarEs聚在“分泌催化类”进化支。CmCarE1、CmCarE2、CmCarE3、CmCarE7、CmCarE8、CmCarE10和CmCarE13特异表达于成虫腹部，而CmCarE9特异表达于雌雄成虫触角。其他基因的表达没有组织特异性。【结论】 CmCarE1、CmCarE2、CmCarE3、CmCarE7、CmCarE8、CmCarE10和CmCarE13编码的酯酶可能参与了内外源化合物的代谢，而CmCarE9编码的酯酶可能参与了气味分子的降解。
英文摘要： [Objectives] To identify carboxylesterase genes (CarEs) in Cnaphalocrocis medinalis and determine their expression profiles in various adult tissues. [Methods] C. medinalis transcriptome datasets were searched to identify CarEs. Bioinformatic software was used to analyze the identified CarE sequences, and a real-time quantitative PCR assay was performed to investigate their relative expression levels. [Results] A total of 15 CarEs were identified from the C. medinalis transcriptome datasets and named CmCarE1-CmCarE15. Of these, CmCarE12 lacked the 3¢-region whereas the other 14 sequences contained complete open reading frames (ORFs). With the exception of CmCarE11, protein encoded by these CmCarE genes showed features typical of CarEs, including a conserved pentapeptide, catalytic triad, and oxyanion hole. Phylogenetic analysis showed that the 15 CmCarEs fell into different clades; CmCarE13, CmCarE14 and CmCarE15 fell into the “intracellular catalytic class”, whereas the other 12 CmCarEs fell into the “secreted catalytic class”. CmCarE1, CmCarE2, CmCarE3, CmCarE7, CmCarE8, CmCarE10 and CmCarE13 were specifically expressed in the adult abdomen, whereas CmCarE9 was specifically expressed in male and female antennae. Other genes were not tissue-specific. [Conclusion] CmCarE1, CmCarE2, CmCarE3, CmCarE7, CmCarE8, CmCarE10 and CmCarE13 might possibly be involved in the metabolism of endobiotic and xenobiotic compounds, whereas CmCarE9 might be involved in the degradation of odorant molecules.