
柑桔全爪螨高致病力菌株芽枝状枝孢霉的生物学特性研究
Biological characteristics of a strain of Cladosporium cladosporioides that is highly pathogenic to Panonychus citri (McGregor)
王 娟** 胡军华*** 龙艳玲 周 娜 彭凤格 姚廷山
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DOI:10.7679/j.issn.2095-1353.2016.003
作者单位:西南大学柑桔研究所∕国家柑桔工程技术研究中心/西南果树科学观测实验站,重庆 400712
中文关键词:芽枝状枝孢霉,柑桔全爪螨,生物学特性
英文关键词:Cladosporium cladosporioides, Panonychus citri, biological characteristic
中文摘要:
明确柑桔全爪螨Panonychus citri(McGregor)高致病力菌株芽枝状枝孢霉Cladosporium cladosporioides(CQBBW3)的生物学特性。【方法】 通过单因素试验,测试了不同培养基、碳源、氮源、温度及pH对CQBBW3菌株生长和产孢的影响。【结果】 CQBBW3菌株的LC50为4.66×108个孢子/mL,LT50为6.2353 d。最佳生长培养基是淀粉琼脂培养基,最适产孢培养基为察氏培养基;菌丝生长最适碳源和氮源为甘露醇和甘氨酸,产孢最适碳源和氮源为乳糖和赖氨酸;最适生长温度为25℃,最适产孢温度为15℃和30℃;最适生长pH值为6.0~8.0,最适产孢pH值7.0。【结论】 CQBBW3菌株对柑桔全爪螨有较好的杀虫活性,喜好25℃、中性、富含有机营养的环境。本研究为菌株快速培养及其真菌杀虫制剂的开发与田间应用奠定基础。
英文摘要:
To explore the biological characteristics of Cladosporium cladosporioides, a newly isolated fungal strain that is highly toxic to Panonychus citri. [Methods] The effects of different factors, such as culture medium, carbon sources, nitrogen sources, temperature, and pH, on the hyphal growth and sporulation of C. cladosporioides, were tested using single factor tests. [Results] The results indicate that the LC50 of the CQBBW3 strain was 4.66×108 spores/mL and the LT50 was 6.2353 d. The optimum culture medium for growing the CQBBW3 strain was Czapek's Medium. Mannitol and Glycine were the best carbon and nitrogen sources for hyphal growth, and Lactose and Lysine were the best carbon and nitrogen sources for sporulation. The most suitable culture temperature for hyphal growth was 25℃, and the most suitable temperatures for sporulation were 15℃and 30℃.The optimum pH for hyphal growth and sporulation were 6.0-8.0 and 7.0, respectively. Although pH had no significant effect on fungal growth rate it had a marked effect on sporulation. [Conclusion] The CQBBW3 strain was toxic to P. citri, and grew best under conditions of 25℃, neutral pH and high organic nutrition. This study provides a scientific basis for both the rapid large-scale culture of the CQBBW3 strain and its effective utilization in the field.