热驯化对禾谷缢管蚜杀虫剂敏感性及解毒酶的影响
The effects of heat acclimation on insecticide susceptibility and detoxification enzyme of Rhopalosiphum padi
李兴烨，李玉婷
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DOI：10.7679/j.issn.2095-1353.2024.086
作者单位：沈阳农业大学植物保护学院
中文关键词：禾谷缢管蚜；热驯化；毒力测定；增效剂；解毒酶
英文关键词：Rhopalosiphum padi; heat acclimation; toxicity assay; synergist; detoxification enzyme
中文摘要：
摘 要 【目的】 明确热驯化（27 ℃）对禾谷缢管蚜Rhopalosiphum padi杀虫剂敏感性及其体内解毒酶的影响。【方法】 采用带虫浸叶法、酶活力测定和实时荧光定量PCR分别测定杀虫剂的敏感性、解毒酶活力和基因表达量。【结果】 热驯化后禾谷缢管蚜对6种杀虫剂的敏感性改变，其中啶虫脒的相对毒力指数最高（4.59）。增效剂试验结果显示，在常温（CK）和热驯化（HA）品系中顺丁烯二酸二乙酯（Diethyl maleate，DEM）对啶虫脒无明显增效作用，而胡椒基丁醚（Piperomyl butoxide，PBO）对啶虫脒增效作用显著，增效比分别为3.95和22.41，但磷酸三苯酯（Triphenyl phosphate，TPP）仅在热驯化品系中对啶虫脒增效作用显著，增效比为4.82。解毒酶活力测定表明，禾谷缢管蚜热驯化品系中细胞色素P450（P450）酶活性显著高于常温品系。定量分析显示，解毒酶基因CarE（2.1，P=0.037）、GSTO（1.7，P=0.035）、CYP6CY7（7.0，P=0.003）和CYP6CZ1（1.9，P=0.013）表达量在热驯化品系中显著上调，而CYP6CJ1（0.40，P=0.026）在热驯化品系中显著下调。【结论】 热驯化显著提高禾谷缢管蚜对啶虫脒的敏感性以及PBO和TPP对啶虫脒的增效作用；热驯化显著提高禾谷缢管蚜体内解毒酶P450活性，且显著诱导5个解毒酶基因的差异表达。
英文摘要：
Abstract [Aim] To investigate the effects of heat acclimation (27 ℃) on insecticide susceptibility and detoxification enzyme activity of Rhopalosiphum padi. [Methods] The leaf-dipping method, enzyme activity assay and real-time quantitative PCR (RT-qPCR) were used to measure insecticide susceptibility, detoxification enzymes activity, and gene expression, respectively, in R. padi. [Results] Heat acclimation had a significant effect on the susceptibility of R. padi to six insecticides, with acetamiprid having the highest index of relative toxicity (4.59). Synergist experiment showed that diethyl maleate (DEM) had no significant synergistic effect on acetamiprid in either the control (CK) or heat acclimation (HA) strains. Conversely, piperonyl buoxide (PBO) had a significant synergistic effect on acetamiprid in both CK and HA strain, with synergism ratios of 3.95 and 22.41, respectively. Moreover, triphenyl phosphate (TPP) had a significant synergistic effect on acetamiprid in the HA strain, with a synergism ratio of 4.82. The activity of cytochrome P450 (P450) in the HA strain was significantly higher than CK strain. RT-qPCR revealed that the expression of CarE (2.1, P=0.037), GSTO (1.7, P=0.035), CYP6CY7 (7.0, P=0.003) and CYP6CZ1 (1.9, P=0.013) was significantly upregulated in the HA strain, while CYP6CJ1 (0.40, P=0.026) was significantly downregulated in the HA strain. [Conclusion] Heat acclimation significantly increased the sensitivity of R. padi to acetamiprid, and the synergism of piperonyl butoxide and triphenyl phosphate on acetamiprid. Heat acclimation significantly increased the activity of detoxifying enzyme P450 and induced the differential expression of five detoxification enzyme genes.