【目的】 复眼作为昆虫重要的光感受器官，在成虫定位导航中起着重要作用。Twin of eyeless (toy)基因位于视网膜决定基因网络的顶端，是昆虫复眼发育中的“主控基因”。以世界性仓储害虫烟草甲Lasioderma serricorne为对象，通过RNA干扰（RNA interference，RNAi）技术阐明Lstoy基因在烟草甲复眼发育过程中的作用。【方法】 通过烟草甲转录组数据筛选获得Lstoy基因，克隆获得其开放阅读框序列并进行生物学信息分析，利用qPCR技术检测Lstoy基因的时空表达模式，通过RNAi抑制烟草甲Lstoy的表达，观察记录沉默目的基因后试虫的发育情况，并检测视网膜决定核蛋白基因和视蛋白基因的表达量变化。【结果】 烟草甲Lstoy基因（GenBank登录号：PP796794.1）的开放阅读框序列长度为1 380 bp，编码459个氨基酸残基，具有pax6蛋白特有保守结构域。Lstoy在烟草甲各发育阶段均有表达，在成虫期和蛹期高表达；Lstoy在5日龄蛹的复眼中表达量最高。Lstoy 基因的RNAi对蛹期Lstoy的表达抑制率为54.73%-77.52%，注射dsLstoy的预蛹45.56%无法完成蜕皮，复眼发育畸形而死亡；42.22%虫体蜕皮延迟2-3 d，复眼色素沉积缓慢，虫体形态较小而死亡；8.89%虫体成功蜕皮至成虫，但鞘翅形成异常，头部黏有部分旧表皮且复眼缩小而死亡。敲低烟草甲Lstoy表达后5个核蛋白基因（Lseya、Lstsh、Lshth、Lsso和Lsey）和2个视蛋白基因（Lslw和Lsuv）的表达量较对照下降43.40%-96.94%。【结论】 Lstoy基因在烟草甲蜕皮变态和复眼发育过程中发挥关键作用。

 [Aim]  To clarify the role of Lstoy gene in the development of the compound eyes of Lasioderma serricorne, a global pest of stored foods. [Methods]  The toy gene was obtained by screening L. serricorne transcriptome data, and the open reading frame (ORF) of Lstoy was cloned and analyzed using bioinformatics. The expression levels of Lstoy were detected in different developmental stages and tissues using quantitative real-time PCR (qPCR). RNAi was used to knockdown the expression of Lstoy, after which any subsequent developmental abnormalities, including changes in the expression levels of retinal determination nuclear, and visual, protein genes were observed and recorded. [Results]  The ORF sequence of Lstoy (GenBank accession number: PP796794.1) is 1 380 bp in length, encodes 459 amino acids and has a conserved domain unique to the pax6 gene. Lstoy was continuously expressed in all life stages tested, with highest expression in the adult and pupal stages. Lstoy had the highest expression level in the compound eyes of 5-day-old pupae. RNAi inhibited the expression of Lstoy in prepupae by 54.73%-77.52%. 45.56% of prepupae injected with dsLstoy were unable to complete molt, resulting in malformed compound eye development and death. In 42.22% of prepupae, injection with dsLstoy resulted in molt being delayed by about 2-3 d, slow pigment deposition in the compound eyes, small body size, and ultimately, death. Only 8.89% of prepupae injected with dsLstoy successfully completed molt, but these had abnormal elytra, old epidermis partially adhered to their heads and shrunken compound eyes. All this group also ultimately died. Knock down of Lstoy significantly decreased the expression of five nuclear protein genes (Lseya, Lstsh, Lshth, Lsso, and Lsey) and two visual protein genes (Lslw and Lsuv) by 43.40%-96.94% compared to the control. [Conclusion]  The Lstoy gene plays a crucial role in the molting, metamorphosis, and compound eye development of L. serricorne.