大蜡螟触角感觉神经元膜蛋白SNMPs的 克隆及组织表达分析
Cloning and expression pattern of sensory neuron membrane protein genes of Galleria mellonella (Lepidoptera: Pyralidae)
谢佼昕1, 2** 张惠满1 孙乐乐1 张 宇1 郭 媛3***
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DOI：10.7679/j.issn.2095-1353.2025.034
作者单位：1. 山西农业大学动物科学学院，太谷 030801；2. 畜禽遗传资源发掘与精准育种山西省重点实验室，太谷 030801； 3. 山西农业大学园艺学院，太原 030031
中文关键词：大蜡螟；感觉神经元膜蛋白；克隆；序列分析；组织表达
英文关键词：Galleria mellonella; sensory neuron membrane protein; cloning; sequence analysis; expression pattern
中文摘要：
【目的】 鉴定并克隆大蜡螟Galleria mellonella触角感觉神经元膜蛋白基因GmelSNMP1和GmelSNMP2，明确GmelSNMP1和GmelSNMP2在大蜡螟成虫不同组织的表达谱，为进一步研究其功能提供理论基础。【方法】 基于大蜡螟成虫触角转录组测序数据，通过PCR克隆具有完整ORF的GmelSNMP1和GmelSNMP2 cDNA序列；利用系统发育进化分析和序列分析研究GmelSNMP1和GmelSNMP2的分类及序列结构特征；采用荧光定量PCR检测该基因在大蜡螟成虫不同组织中的表达水平。【结果】 克隆得到大蜡螟GmelSNMP1和GmelSNMP2（GenBank登录号：PQ824699和PQ824700）的cDNA全长序列；GmelSNMP1基因ORF含有1 578个核苷酸，编码525个氨基酸；GmelSNMP2基因ORF含有1 566个核苷酸，编码521个氨基酸。GmelSNMP1和GmelSNMP2蛋白N端位于胞内的2个跨膜域和1个大的胞外环状结构域，与脊椎动物CD36蛋白家族具有相似的结构特点。GmelSNMP1主要在雌雄触角中表达，且雌虫触角表达量显著高于雄虫触角（P=0.002）；而GmelSNMP2在触角和其他组织中均有表达。【结论】 该研究结果为进一步探索大蜡螟SNMPs在检测性信息素和一般气味分子中的功能提供了理论基础。
英文摘要：
[Aim] To clone the sensory neuron
membrane protein (SNMP) genes, GmelSNMP1 and GmelSNMP2, in the
antennae of the wax moth, Galleria mellonella, and clarify the
expression profiles of these genes in different adult tissues, and thereby
provide a foundation for further research on their function. [Methods] Based on adult antennal transcriptome
sequencing data and the identification of G. mellonella SNMPs, the cDNA
sequence of GmelSNMP1 and GmelSNMP2, including a complete ORF, was
cloned using PCR. Phylogenetic and sequence analysis were used to determine the
classification and sequence structure of these genes, and fluorescent,
quantitative PCR was used to detect their expression in different adult
tissues. [Results] Full-length
cDNA sequences of GmelSNMP1 and GmelSNMP2 (GenBank accession no.:
PQ824699 and PQ824700) were successfully cloned. The ORF of the GmelSNMP1 gene contains 1 578 nucleotides, encoding 525 amino acids; The ORF of the GmelSNMP2 gene contains 1 566 nucleotides, encoding 521 amino acids. The N-terminus of GmelSNMP1 and GmelSNMP2 proteins are in two transmembrane domains and a large
extracellular circular domain within the cell, sharing similar structural
characteristics with the vertebrate CD36 protein family. GmelSNMP1 was
predominantly expressed in the antennae, and its expression in the antennae of
adult females was significantly higher than in males (P=0.002). GmelSNMP2 was expressed in the antennae of both females and males, as well as in
non-antennal tissues. [Conclusion] These results lay a foundation for further
exploring the function of large wax moth SNMPs in detecting sex pheromones and
other odor molecules.