两种赤眼蜂抗/耐药种群P450基因的表达模式分析
Insecticide induced expression of P450 genes in resistant and tolerant populations of Trichogramma japonicum and Trichogramma chilonis
鲁艳辉1** 谢连城1** 杨亚军1 徐红星1 臧连生2 吕仲贤1*** 田俊策1***
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DOI：10.7679/j.issn.2095-1353.2025.067
作者单位：1. 农产品质量安全全国重点实验室，浙江省农业科学院植物保护与微生物研究所，杭州 310021； 2. 绿色农药全国重点实验室，贵州大学，贵阳 550025
中文关键词：稻螟赤眼蜂；螟黄赤眼蜂；抗/耐药性；P450；基因表达
英文关键词：Trichogramma japonicum; Trichogramma chilonis; insecticide resistance/tolerance; P450; gene expression
中文摘要：
【目的】 利用赤眼蜂防治水稻害虫具有绿色、安全、环保等优点。但化学防治与生物防治均用于水稻害虫防治，释放到田间的赤眼蜂不可避免与农药接触。本文研究旨在提高赤眼蜂抗/耐药性，使其更好地发挥对田间害虫控制作用。【方法】 利用荧光定量PCR（RT-qPCR）技术分别测定了稻螟赤眼蜂Trichogramma japonicum和螟黄赤眼蜂T. chilonis对吡虫啉、噻虫嗪、噻嗪酮和烯啶虫胺的抗/耐药种群P450基因表达量。【结果】 稻螟赤眼蜂所有抗/耐药种群不同P450基因均呈现不同程度的差异表达。其中，耐噻虫嗪稻螟赤眼蜂种群CYP4C1和CYP4G15基因显著上调表达；耐噻嗪酮稻螟赤眼蜂种群CYP4C1、CYP6A2和CYP9E2基因显著上调表达；烯啶虫胺抗性稻螟赤眼蜂种群CYP4C1、CYP4G15、CYP6A14和CYP6K1基因显著上调表达。螟黄赤眼蜂吡虫啉抗性种群CYP4AA1、CYP6A2、CYP9AG4、CYP9E2、CYP9P3、CYP307A1和CYP315A1基因显著上调表达；噻虫嗪抗性种群CYP9AG3基因显著上调表达；噻嗪酮耐性种群CYP4AA1、CYP6A2、CYP9E2和CYP307A1基因显著上调表达；烯啶虫胺抗性种群CYP4G43、CYP9P3和CYP307A1基因显著上调表达。【结论】 两种不同赤眼蜂对不同药剂产生抗/耐药性后，分别引起了不同P450基因表达量的变化，初步明确了赤眼蜂的抗/耐药性机制，为抗/耐药性赤眼蜂的田间应用奠定了理论基础。
英文摘要：
[Aim] To determine the effect of exposure to
commonly used insecticides on expression of P450 genes in the wasps Trichogramma japonicum and Trichogramma chilonis, in order to further
understanding of the mechanisms responsible for insecticide tolerance in these
species, and promote their use for pest management purposes. [Methods] Expression of the P450 genes of T.
japonicum and T. chilonis populations that were either resistant,
ortolerant , to imidacloprid, thiothiazide, thiazone and nitenpyram was determined
using RT-qPCR. [Results] The
expression of several P450 genes varied across populations of T. japonicum.
Among these, CYP4C1 and CYP4G15 were significantly upregulated in
thiothiazide-tolerant populations, CYP4C1, CYP6A2, and CYP9E2 in thiazone-resistant populations, and CYP4C1, CYP4G15, CYP6A14,
and CYP6K1 in nitenpyram-resistant populations of T. japonicum. CYP4AA1, CYP6A2, CYP9AG4, CYP9E2, CYP9P3, CYP307A1,
and CYP315A1 were upregulated in imidacloprid-tolerant populations of T.
chilonis, CYP9AG3 in thiothiazide-tolerant populations of T.
chilonis, and CYP4AA1, CYP6A2, CYP9E2, and CYP307A1 in nitenpyram-tolerant populations of T. chilonis. [Conclusion] These results show that two Trichogramma species
from resistant, or tolerant, populations differ in P450 gene expression, a
finding that improves understanding of the mechanism underlying pesticide
resistance in these species, and lays a solid theoretical foundation for the
release of resistant, or tolerant, Trichogramma wasps for biological
control.