【目的】 保幼激素酸甲基转移酶（Juvenile hormone acid O-methyltransferase, JHAMT）是参与昆虫保幼激素合成的关键限速酶。本研究旨在鉴定和表达亚洲玉米螟Ostrinia furnacalis JHAMT基因，揭示其在亚洲玉米螟幼虫滞育中的作用。【方法】 基于本实验室前期组装的亚洲玉米螟转录组数据（NCBI登录号：PRJNA1030200），采用RT-PCR技术克隆鉴定JHAMT基因并进行生物信息学分析，通过实时荧光定量PCR（qPCR）技术分析其在亚洲玉米螟幼虫不同滞育状态的表达水平，利用dsRNA注射法干扰滞育幼虫的JHAMT基因，探究该基因在亚洲玉米螟滞育过程中的功能。【结果】 亚洲玉米螟OfJHAMT基因开放阅读框长度为948 bp，编码315个氨基酸，预测蛋白质分子量为36.40kD，等电点pI为7.64。同源序列比对和系统发育分析表明，亚洲玉米螟OfJHAMT与欧洲玉米螟Ostrinia nubilalis 的OnJHAMT亲缘关系最近，其氨基酸序列一致性为96.51%。OfJHAMT基因在亚洲玉米螟的不同滞育阶段均有表达，其中滞育期的相对表达量最低，解除滞育后表达量升高。RNAi实验结果表明，注射dsOfJHAMT 48 h后OfJHAMT基因表达量下调86.35%。沉默OfJHAMT基因后第9天，亚洲玉米螟的存活率降至56.67%，与对照组dsGFP相比，滞育率下降了81.67%，且试验组幼虫不同程度出现外表皮变黑，组织软化及皱缩等致死表型，存活个体多生长为畸形蛹，无法正常发育成成虫。【结论】 本研究成功鉴定了亚洲玉米螟OfJHAMT基因，其在不同滞育状态下的表达模式呈现显著性差异表达，OfJHAMT表达被降低后影响幼虫生长发育且滞育率显著低于对照组，表明OfJHAMT基因在亚洲玉米螟滞育过程中起着重要作用。

 [Aim]  To identify the Ostrinia furnacalis juvenile hormone acid O-methyltransferase (OfJHAMT) gene, and to investigate its role in diapause in this species. [Methods]  Based on O. furnacalis transcriptome data previously obtained by our lab (NCBI accession number : PRJNA1030200 ), the OfJHAMT gene was cloned and identified with RT-PCR and analyzed using bioinformatics. The expression of the OfJHAMT gene in different diapause stages of O. furnacalis larvae was analyzed using real-time fluorescent quantitative PCR (qPCR), and its function during the diapause process was investigated using RNA interference (RNAi; injecting dsRNA into diapausing larvae). [Results]  The open reading frame (ORF) of the OfJHAMT gene is 948 bp long, encodes 315 amino acids, and has a predicted protein molecular weight of 36.40 kD and an isoelectric point (pI) of7.64. Homologous sequence alignment and phylogenetic analysis indicate that OfJHAMT is most closely related to the OnJHAMT gene of the European corn borer, Ostrinia nubilalis, with which it has an amino acid sequence identity of 96.51%. The OfJHAMT gene was expressed in all diapause stages, with the lowest relative expression during the diapause period. Expression levels increased after diapause ended. The RNAi experiment indicated that expression of OfJHAMT was downregulated by 86.35% 48 h after the injection of dsRNA. The survival rate of larvae dropped to 56.67% by the 9th day after injection, and, compared to the dsGFP control group, the diapause rate of the treatment group was 81.67% lower. In addition, larvae treated with dsRNA exhibited different degrees of cuticle darkening, tissue softening, and wrinkling, phenotypes that were often lethal, with surviving individuals mostly being deformed pupae that were unable to develop normally into adults. [Conclusion]  The OfJHAMT gene appears to play an important role in the diapause process of O. furnacalis larvae.