【目的】 旨在探究lncRNA17000在意大利蜜蜂Apis mellifera ligustica工蜂不同组织和发育阶段中的表达模式及其调控作用，为进一步的功能和机制研究提供基础。【方法】 通过RT-PCR验证lncRNA17000在工蜂不同组织中的表达。采用RT-qPCR检测其不同组织和发育阶段的相对表达量。利用LncTar、Miranda、RNAhybrid和TargetScan等一系列软件分析lncRNA17000的顺式、反式和竞争性内源RNA（Competing endogenous RNA, ceRNA）调控作用。【结果】 在工蜂的脑、触角、毒腺、中肠、表皮、咽下腺和脂肪体7个组织中均扩增出符合预期大小（约133 bp）的目的片段。LncRNA17000在上述7个组织中差异表达，在触角中的表达量最高且显著高于（P<0.05）脂肪体中的表达量。LncRNA17000在工蜂的卵、幼虫、预蛹、蛹和成虫中差异表达，在7日龄预蛹中的表达量最高且显著高于（P<0.05）3日龄幼虫、8日龄预蛹和12日龄蛹中的表达量。LncRNA17000在6、12、15和18日龄成虫体内的表达量显著低于（P<0.05）1日龄成虫体内的表达量且表达水平随日龄增长而降低。LncRNA17000潜在调控7个上下游基因的转录和2个共表达基因的表达。LncRNA17000可靶向45个miRNA进而靶向66条mRNA，这些靶mRNA涉及23个GO条目和25条KEGG通路。【结论】 LncRNA17000在意大利蜜蜂工蜂的不同组织和发育阶段中动态差异表达，在触角和7日龄预蛹中特异性高表达；lncRNA17000可能通过顺式作用调控TFIID亚基11亚型X2基因转录，通过反式作用调控蜜蜂翼视蛋白基因表达，通过ceRNA网络靶向多个miRNA和mRNA进而影响胰岛素、ErbB和mTOR等信号通路。

  [Aim]  To investigate the expression of the lncRNA17000 gene in different tissues and developmental stages of Apis mellifera ligustica to provide a foundation for further functional and mechanistic studies of this gene. [Methods]  The expression of lncRNA17000 in various tissues was verified by RT-PCR, and its relative expression in different tissues and developmental stages was quantified using RT-qPCR. The cis, trans and competitive, endogenous RNA regulatory roles of lncRNA17000 were analyzed using relevant software. [Results]  Target fragments of the expected size were amplified in seven tissues of worker bees, including the brain, antennae, venom glands, midgut, epidermis, hypopharyngeal glands, and fat body. LncRNA17000 was differentially expressed in the above seven tissues, with significantly higher expression in the antennae than in the fat body (P<0.05). LncRNA17000 was also differentially expressed in eggs, larvae, prepupae, pupae, and adults, with significantly higher expression in 7-day-old prepupae than in 3-day-old larvae, 8-day-old prepupae, and 12-day-old pupae (P<0.05). LncRNA17000 expression in 6, 12, 15 and 18-day-old adults was significantly lower than in 1-day-old adults (P<0.05), and its expression decreased with age. LncRNA17000 potentially regulates the transcription of seven upstream and downstream genes and the expression of two co-expressed genes. It targets 45 miRNAs, which, in turn, target 66 mRNAs involved in 23 GO terms and 25 KEGG pathways. [Conclusion]  LncRNA17000 is widely expressed in several tissues and developmental stages of A. m. ligustica, but was most highly expressed in the antennae, 7-day-old prepupae and 1-day-old adults. LncRNA17000 is likely to regulate the transcription of the TFIID subunit 11 transcript variant X2 gene in a cis-like manner, and to modulate the expression of the pteropsin gene in a trans-like manner. It likely targets a series of miRNAs and mRNAs, thereby further regulating the insulin, ErbB and mTOR signaling pathways.