【目的】 明确柑桔红蜘蛛Panonychus citri敏感种群对果园常用杀虫杀螨剂的敏感性水平及差异，并建立柑桔红蜘蛛对这些杀虫杀螨剂的敏感基线，最终形成柑桔红蜘蛛田间种群药剂敏感性的快速诊断方法，以期在柑桔红蜘蛛的化学防治中提供精准选药的理论科学依据。【方法】 采用叶片浸渍法测定10种杀虫杀螨剂对柑桔红蜘蛛敏感品系的毒力水平，并用死亡概率值法计算得到LC₅₀和LC₉₉值。以2×LC₉₉值作为诊断剂量备选浓度，并设置相应的诊断剂量浓度梯度进行验证试验，选取柑桔红蜘蛛死亡率为100%的浓度值作为诊断剂量浓度。根据毒力回归曲线和诊断剂量法制定柑桔红蜘蛛诊断试剂盒敏感性等级评价标准。【结果】 10种杀虫杀螨剂的诊断剂量阿维菌素、丁氟螨酯、联苯肼酯、乙螨唑、螺螨酯、螺虫乙酯、炔螨特、哒螨灵、毒死蜱和苦参碱分别为550、400、700、650、3 300、2 000、850、350、550和950 mg/L。诊断试剂盒抗性评价标准为死亡率≥ 95%，敏感状态；死亡率88%-95%，敏感性下降状态；死亡率< 88%，非敏感状态。【结论】 研究所得诊断剂量浓度可以通过测定柑桔红蜘蛛田间种群死亡率，快速评估柑桔红蜘蛛田间种群对杀虫杀螨剂的敏感水平，为今后快速、精准选择高效的杀虫杀螨剂而指导田间用药提供了新方法。

 [Aim]  To develop a relatively quick method to detect the sensitivity of Panonychus citri to commonly used insecticides and acaricides in orchards, thereby optimizing the use of these chemicals. [Methods]  The toxicity of 10 insecticides and acaricides to sensitive strains of P. citri were determined using the leaf impregnation method. LC₅₀ and LC₉₉ values were calculated using the probability of death method. Using twice the LC₉₉ value as the alternative diagnostic concentration, and setting a corresponding diagnostic concentration gradient for validation experiments, the concentration that achieved 100% mortality was selected as the diagnostic dose. Based on the toxicity regression curve and diagnostic dose method, evaluation criteria for pesticide sensitivity for a P. citri diagnostic kit were developed. [Results]  The diagnostic doses of 10 insecticides and acaricides, abamectin, cyflumetofen, bifenazate, etoxazole, spirodiclofen, spirotetramat, progargite, pyridaben, chlorpyrifos and matrine, were 550, 400, 700, 650, 3 300, 2 000, 850, 350, 550 and 950 mg/L, respectively. The diagnostic kit classifies populations in terms of resistance as follows; those with a mortality rate ≥ 95% are classified as sensitive, those with a mortality rate between 88% and 95% are classified as having reduced sensitivity, and those with a mortality rate < 88% are classified as resistant. [Conclusion]  The diagnostic kit developed in this study can quickly assess the sensitivity of P. citri populations to insecticides and acaricides in the field by measuring the mortality rate. This provides a way to quickly select the most effective insecticides and acaricides for controlling a given P. citri population at a particular point in time.