【目的】 长白山中华蜜蜂Apis cerana cerana in Changbai Mountain是在长白山独特的自然生态环境中，历经长期的自然选择压力而形成的东方蜜蜂Apis cerana的一个生态类型，对当地冬季严寒，无霜期短等严酷自然环境具有显著的生态适应性。本研究旨在探究越冬期特定环境下长白山中华蜜蜂耐寒适应性相关的代谢物和代谢通路。【方法】 以越冬中期的长白山中华蜜蜂为研究对象，采用液相色谱-质谱联用技术（Liquid chromatography mass spectrometry, LC-MS）对比越冬期（12月）和非越冬期（8月）工蜂肠道组织中代谢物的变化。【结果】 越冬蜂和非越冬蜂个体在正、负离子模式下分别获得125和219个差异代谢物，在正、负离子模式下最显著差异的代谢物中筛选得到褪黑素、异鼠李素、飞燕草素、亚油酸、亚麻酸、喹啉酸、犬尿氨酸、硫胺素、吡哆醇和紫丁香苷等多种与蜜蜂抗氧化损伤、抗微生物侵染、抗寒、营养和能量物质的合成与代谢相关的差异代谢物。差异代谢物显著富集于嘌呤代谢、磷酸戊糖途径、糖基磷脂酰肌醇（Glycosylphosphatidylinositol，GPI）锚生物合成以及硫中继系统、烟酸和烟酰胺代谢、丙氨酸、天冬氨酸和谷氨酸代谢等KEGG通路。【结论】 长白山中华蜜蜂主要通过调节抗氧化代谢和能量物质合成代谢应对越冬期逆境胁迫，研究结果初步阐释了长白山中华蜜蜂耐寒适应性的代谢特点和调控基础。

 [Aim]  To investigate the metabolites and metabolic pathways related to cold-resistance in the honeybee Apis cerana cerana, an ecological type of A. cerana native to the Changbai Mountains, during the overwintering period. [Methods]  Changes in metabolites in the guts of overwintering (December) and non-overwintering (August) honeybees were detected using a non-targeted metabolomics methods based on liquid chromatography mass spectrometry. [Results]  125 positive and 219 negative ion mode, differentially expressed metabolites were detected in overwintering and non-overwintering honeybees. Of these, 180 were upregulated and 164 were downregulated. The VIP top 30 metabolites included various differential metabolites, many of which appear related to reducing antioxidant damage, antimicrobic activity, cold resistance, synthesis and the metabolism of nutrients or energy substances, such as melatonin, isorhamnetin, delphinidin, linoleic acid, Y-linolenic acid, quinolinic acid, kynurenine, thiamine, pyridoxine, and syringin. The most significant difference was among the positive and negative ion modes. In addition, the purine metabolism, pentose phosphate pathway, glycosylphosphatidylinositol (GPI) anchor biosynthesis, as well as the sulfur relay system, nicotinate and nicotinamide metabolism, alanine, aspartic acid, and glutamate metabolism, were the most significantly downregulated and upregulated KEGG pathways. Differential metabolites were significantly enriched in KEGG pathways such as the purine metabolism, pentose phosphate pathway, glycosylphosphatidylinositol (GPI) anchor biosynthesis, sulfur relay system, nicotinate and nicotinamide metabolism, alanine, aspartic acid, and glutamate metabolism. [Conclusion]  A. c. cerana mainly responds to overwintering stress by regulating its antioxidant metabolism, energy substance synthesis and metabolic rate. These results shed light on the metabolic characteristics and regulatory basis of cold-resistance in A. c. cerana in the Changbai Mountains.