【目的】 日本松干蚧Matsucoccus matsumurae作为外来入侵害虫，对松林的健康和生态安全构成了严重威胁。本研究旨在探索日本松干蚧不同抗性寄主次生代谢和挥发性物质差异，为今后探索化学生态诱导、寻找环境友好型杀虫剂、开发影响昆虫行为的化学信息物质等防控日本松干蚧的应用提供理论依据。【方法】 本研究通过靶向代谢物检测和动态顶空进样GC/MS法对6种松树易被日本松干蚧寄生部位的非挥发性次生代谢酚类和挥发性次生代谢有机物的种类和含量进行研究分析。【结果】 易被日本松干蚧寄生部位的枝条树皮内的单宁和酚酸，尤其是儿茶素和表儿茶素与树种抗性呈正相关；抗性树种樟子松Pinus sylvestris特有且相对含量大的挥发性有机物有(+)-柠檬烯、莰烯、3-蒈烯和γ-杜松烯，感性树种赤松P. densiflora中相对含量较大的有月桂烯和水芹烯。【结论】 儿茶素和表儿茶素可作为判断日本松干蚧抗性树种的指示性指标。(+)-柠檬烯、莰烯、3-蒈烯、γ-杜松烯、月桂烯和水芹烯等不同抗性树种的特异性挥发物是对日本松干蚧具有驱避或引诱效果的化合物。

[Aim]  To explore the differences in secondary metabolites and volatile organic compounds among different resistant hosts of Matsucoccus matsumurae, and to identify eco-friendly insecticides and semiochemicals that influence insect behavior, with the aim of improving control strategies against M. matsumurae. This exotic invasive pest poses serious threats to pine forest health and ecological security. [Methods]  Non-volatile secondary metabolites (phenolic compounds) and volatile organic compounds were identified and analyzed in six pine species with varying resistance to M. matsumurae infestation, using targeted metabolomics and dynamic headspace GC/MS. [Results]  Tannins and phenolic acids, particularly catechins and epicatechins, in the bark of branches that are easily parasitized by M. matsumurae are positively correlated with resistance to this pest. A resistant species, Pinus sylvestris, has unique and relatively high levels of volatile organic compounds such as (+)-limonene, camphene, 3-carene, and γ-cadinene, whereas a susceptible species, P. densiflora, contains relatively higher levels of myrcene and phellandrene. [Conclusion]  Catechins and epicatechins can indicate whether a pine species is resistant to M. matsumurae. The specific volatile compounds found in different resistant tree species, such as (+)-limonene, camphene, 3-carene, γ-cadinene, myrcene, and phellandrene, may be attractive, or repellent, to M. matsumurae.