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豇豆荚螟幼虫对常用杀虫剂的 相对敏感基线建立
Determining the baseline susceptibility of Maruca vitrata (Lepidoptera: Crambidae) larvae to commonly used insecticides
周哲清1, 2** 周利琳1 望 勇1 杨 帆1 杨绍丽1 蔡 翔1 骆海波1 司升云1 王 攀1***
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DOI:10.7679/j.issn.2095-1353.2025.102
作者单位:1. 武汉市农业科学院蔬菜研究所,武汉 430345;2. 华中农业大学植物科学技术学院,武汉 430070
中文关键词:豇豆荚螟;杀虫剂;饲料药膜法;饲料混药法;敏感基线
英文关键词: Maruca vitrata; insecticides; diet surface overlay bioassay; diet incorporation bioassay; susceptible baseline
中文摘要:

【目的】 通过2种生物测定方法评估不同类型化学杀虫剂对豇豆荚螟Maruca vitrata幼虫毒力,建立豇豆荚螟对常用杀虫剂的相对敏感基线,为豇豆荚螟的抗药性监测及抗药性治理提供参考。【方法】 在室内采用饲料药膜法和饲料混药法,分别测定豇豆荚螟3龄幼虫对5大类共8种常用药剂(氯虫苯甲酰胺、溴氰虫酰胺、茚虫威、甲氨基阿维菌素苯甲酸盐、阿维菌素、乙基多杀菌素、虱螨脲和溴虫氟苯双酰胺)的敏感性,并据此建立豇豆荚螟幼虫对8种杀虫剂的相对敏感基线。【结果】 饲料药膜法测得上述8种药剂对豇豆荚螟幼虫的LC50范围为0.007-1.413 μg/cm2;饲料混药法测得上述8种药剂对豇豆荚螟幼虫的LC50范围为0.025-4.178 mg/L。相比饲料药膜法,饲料混药法的χ2值较小、P值较大、种群整体在遗传学上的纯度较高、试验结果的可重复性较好,更适合用于豇豆荚螟对常用药剂的生物活性测定(茚虫威除外)。【结论】 采用饲料药膜法和饲料混药法分别建立了豇豆荚螟幼虫对8种常用杀虫剂的相对敏感基线,为系统开展豇豆荚螟的抗药性监测以及抗性治理提供了参考。

英文摘要:

 [Aim]  Two bioassay methods were used to evaluate the toxicities of different types of insecticides against Maruca vitrata larvae, and to establish the relative susceptibility baselines of M. vitrata to commonly used insecticides, in order to provide reference for systematically resistance monitoring and management of M. vitrata in China. [Methods]  The susceptibility of the 3rd instar larvae of M. vitrata to eight commonly used insecticides (chlorantraniliprole, cyantraniliprole, indoxacarb, emamectin benzoate, abamectin, spinetoram, lufenuron, broflanilide) belonging to five insecticide categories were determined by diet surface overlay bioassay method and diet incorporation bioassay method, respectively. Based on the bioassay results, the relative susceptible baselines of M. vitrata to these eight insecticides were established. [Results]  The LC50 values of the above eight insecticides to M. vitrata larvae were 0.007-1.413 μg/cm2 by diet surface overlay bioassay, and 0.025-4.178 mg/L by diet incorporation bioassay, respectively. Compared with the diet surface overlay bioassay method, the bioassay data obtained in diet incorporation bioassay method had smaller chi-square value and larger P-value. At the same time, M. vitrata populations showed higher genetic purity of the population in the diet incorporation bioassay method than in the diet surface overlay bioassay method, and also showed the highest repeatability of bioassay results. So it is the recommended bioassay method of these commonly used insecticides against M. vitrata larvae (except indocarb). [Conclusion]  The relative susceptibility baselines of M. vitrata larvae to eight commonly used insecticides have been established using two different bioassay methods respectively, providing reference for the resistance monitoring and chemical control of M. vitrata in China.

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