【目的】 探究亚致死浓度阿维菌素、吡虫啉和高效氯氰菊酯对螟黄赤眼蜂Trichogramma chilonis寄生功能及体内解毒酶、保护酶的活性及线粒体呼吸链复合物Ⅰ（Mitochondrial respiratory chain complexⅠ，MRCCⅠ）、三磷酸腺苷（Adenosine triphosphate，ATP）、活性氧（Reactive oxygen species, ROS）、丙二醛（Malondialdehyde，MDA）等物质含量的影响，以阐明3种杀虫剂对螟黄赤眼蜂亚致死效应的作用机制。【方法】 采用药膜法，以亚致死浓度 LC₅₀处理螟黄赤眼蜂，比较不同杀虫剂对螟黄赤眼蜂搜寻效应及处置时间、寄生效能和最大寄生量等寄生能力的影响，并分析相关酶活性以及MRCCⅠ与线粒体呼吸过程中ATP含量的变化。【结果】 阿维菌素、吡虫啉和高效氯氰菊酯处理螟黄赤眼蜂后，搜寻效应均降低；寄生效能分别降低了54.81%、20.79%和11.55%。3种杀虫剂处理后，螟黄赤眼蜂体内的谷胱甘肽S-转移酶（Glutathione S-transferase，GSTs）、细胞色素CYP450（Cytochrome P450，CYP450）、羧酸酯酶（Carboxylesterase，CarE）和过氧化物酶（Peroxidase，POD）活性均显著升高（阿维菌素：P = 0.043、P = 0.005、P = 0.022和P = 0.004、吡虫啉：P < 0.001、P = 0.013、P = 0.017和P < 0.001、高效氯氰菊酯：P < 0.001、P = 0.013、P = 0.002和P = 0.002）。其中阿维菌素处理后ROS（P = 0.050）、MDA（P < 0.001）和MRCCⅠ（P = 0.006）含量显著增加，超氧化物歧化酶（Superoxide dismutase，SOD）活性显著降低（P = 0.050）；吡虫啉处理后MDA（P = 0.010）和MRCCⅠ（P = 0.005）含量显著增加；高效氯氰菊酯处理后过氧化氢酶（Catalase，CAT）（P = 0.034）活性、MRCCⅠ（P = 0.025）、ATP（P = 0.008）、ROS（P = 0.020）和MDA（P = 0.004）含量显著增加。【结论】 3种杀虫剂亚致死浓度均可显著抑制螟黄赤眼蜂的搜寻效应和寄生能力，诱导螟黄赤眼蜂体内解毒酶活性上调，影响螟黄赤眼蜂体内保护酶活性及MRCCⅠ、ATP、ROS和MDA等物质含量。

 [Aim]  To investigate the effects of sublethal concentrations of the insecticides abamectin, imidacloprid and beta-cypermethrin on the parasitic behavior of T. chilonis, on  the activity detoxification and protective enzymes in this species, and on levels of mitochondrial respiratory chain complexⅠ(MRCCⅠ), adenosine triphosphate (ATP), reactive oxygen species (ROS) and malondialdehyde (MDA). [Methods]  The effects of sublethal concentrations of the different insecticides on the prey searching, processing time, parasitic efficiency, and maximum parasitic capacity, of T. chilonis were measured and compared, and changes in enzyme activity, MRCCⅠand ATP content were analyzed using the dry film residue method. [Results]  Abamectin, imidacloprid and beta-cypermethrin significantly reduced the effectiveness of prey searching, and reduced overall parasitic efficiency by 54.81%, 20.79% and 11.55%, respectively. Glutathione S-transferase (GSTs), cytochrome P450 (CYP450), carboxylesterase (CarE) and peroxidase (POD) activity all significantly increased after treatment with the three insecticides (abamectin：P = 0.043, P = 0.005, P = 0.022 and P = 0.004, imidacloprid：P < 0.001, P = 0.013, P = 0.017 and P < 0.001, beta-cypermethrin：P < 0.001, P = 0.013, P = 0.002 and P = 0.002). ROS (P = 0.050), MDA (P < 0.001) and MRCCI (P = 0.006) content significantly increased after treatment with abamectin. Superoxide dismutase (SOD) activity significantly decreased (P = 0.050), whereas MDA (P = 0.010) and MRCCI (P = 0.005) content significantly increased after treatment with imidacloprid. Catalase (CAT) (P = 0.034), ROS (P = 0.025), MDA (P = 0.008), MRCCI (P = 0.020) and ATP (P = 0.004) content significantly increased after beta-cypermethrin treatment. [Conclusion]  Sublethal concentrations of abamectin, imidacloprid and beta-cypermethrin, significantly reduced the prey searching efficiency and parasitic ability of T. chilonis (parasitic ability：20 grain：P = 0.004; 30 grain：P < 0.001; 50 grain：P < 0.001; 60 grain：P < 0.001; 100 grain：P = 0.001; 120 grain：P = 0.003; 150 grain：P = 0.001). Treatment with these pesticides also increased detoxification enzyme activity, and affected both protective enzyme activity and ROS, MDA, MRCCⅠand ATP content, in this species.