
黄粉虫幼虫对不同调味料挥发物的嗅觉选择反应
Olfactory responses of Tenebrio molitor larvae to different plant volatiles
张萌昊1** 刘晓光1, 2 王 昆3 郭线茹1, 2 张利娟1, 2 刘艳敏1, 2 王高平1, 2 李为争1, 2***
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DOI:10.7679/j.issn.2095-1353.2026.010
作者单位:1. 河南农业大学植物保护学院, 郑州 450002;2. 河南省害虫绿色防控国际联合实验室, 河南农业大学,郑州 450002;3. 武汉乐道物流有限公司,武汉 430000
中文关键词:黄粉虫;调味料;嗅觉选择;反式茴香脑
英文关键词:Tenebrio molitor; flavorant; olfactory choice; trans-Anethole
中文摘要:
【目的】 筛选对黄粉虫Tenebrio
molitor幼虫有行为调控活性的调味料种类,为黄粉虫饲养或防除提供科学依据。【方法】 采用Y形嗅觉仪测试了黄粉虫幼虫对12种调味料的嗅觉选择反应,对八角Illicium
verum粗提物进行GC-MS分析,用八角主成分制备了3个配方进行生物测定,并测试了黄粉虫幼虫对反式茴香脑的剂量反应。【结果】 12种材料的提取物中,唯有八角提取物对黄粉虫幼虫具有显著引诱活性(P=0.005 3),该提取物测试时,选择处理、对照以及不反应的虫数分别为30、3和7头。八角提取物的主要成分是反式茴香脑(65.46%)、芳樟醇(7.00%)、(E)-b-石竹烯(4.58%)和(Z,E)-a-法尼烯(4.48%)。经过配方测试发现,反式茴香脑单独可以解释八角挥发物对黄粉虫幼虫的引诱活性。然而,黄粉虫幼虫对反式茴香脑的行为学剂量反应测试发现,该物质在0-5 mL的剂量范围内,存在“低剂量引诱、高剂量驱避”的特征。【结论】 低剂量反式茴香脑可作为饲料添加剂,用于改善黄粉虫人工饲料的嗅感特征。
英文摘要:
[Aim] To determine
the attractiveness of the volatile compounds of different spices to Tenebrio molitor larvae, thereby
providing a scientific basis for the rearing or control of this pest. [Methods] Olfactory responses of larvae to 12 spices
were tested in a Y-tube olfactometer. Crude extract of Illicium verum (star anise) was analyzed by GC-MS, and three blends
based on its primary components were tested. The dose-response curves of trans-Anethole
were quantified. [Results] Among the 12 spice extracts tested, only star anise extract was
significantly attractive; 30 larvae preferred it to the control, 3 preferred the control and 7 had no obvious preference
(P = 0.005 3). Gas
chromatography-mass spectrometry analysis identified trans-Anethole (65.46%),
linalool (7.00%), (E)-β-caryophyllene (4.58%), and (Z,E)-α-farnesene
(4.48%), as the major components of the star anise extract. Of the three
volatile blends tested, only trans-Anethole was as attractive to larvae
as natural star anise extract. Dose-response assays revealed that within a
range of doses from 0 to 5 mL, trans-Anethole was attractive to larvae at low doses and
repellent at high doses. [Conclusion]
Low-dose trans-Anethole may be
used as a feed additive to improve the olfactory characteristics of diets for T. molitor larvae.