渐狭蜡蚧菌对西花蓟马卵黄原蛋白的影响
The effect of Lecanicillium attenuatum on vitellogenin in Frankliniella occidentalis
张 洋** 谢 文** 郅军锐*** 周叶鸣 李定银 庹 露
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DOI：10.7679/j.issn.2095-1353.2026.011
作者单位：贵州大学昆虫研究所，贵州省山地农业病虫害重点实验室，贵阳 550025
中文关键词：西花蓟马；渐狭蜡蚧菌；卵黄原蛋白；生殖；生物防治
英文关键词： Frankliniella occidentalis; Lecanicillium attenuatum; vitellogenin; reproduction; biological control
中文摘要：
【目的】 明确渐狭蜡蚧菌Lecanicillium
attenuatum对西花蓟马Frankliniella occidentalis卵黄原蛋白（Vg）的影响，为西花蓟马的生物防治提供理论依据。【方法】 采用浸虫法研究了渐狭蜡蚧菌单次和多次侵染西花蓟马2龄若虫后对F1-F3代雌成虫卵黄原蛋白表达量和含量的影响。【结果】 在两性生殖方式下，渐狭蜡蚧菌单次侵染西花蓟马2龄若虫时，只有F2代Vg表达量显著高于对照（P<0.05）；渐狭蜡蚧菌多次侵染后，只有F1代Vg表达量明显高于对照（P<0.05）。在孤雌生殖方式下，不论单次侵染还是多次侵染，F2和F3代Vg表达量均明显高于对照（P<0.05）。在两性生殖方式下，不论渐狭蜡蚧菌单次侵染还是多次侵染，F1和F2代的Vg含量均显著高于对照（P<0.05）；在孤雌生殖方式下，单次侵染处理后F1和F2代Vg含量均显著高于对照（P<0.05），多次侵染处理下3个世代均显著升高（P<0.05）。【结论】 渐狭蜡蚧菌侵染显著影响西花蓟马卵黄原蛋白的表达量及其含量，且影响程度与侵染次数、蓟马的世代及生殖方式有关。
英文摘要： [Aim] To
clarify the effect of infection by Lecanicillium
attenuatum on the vitellogenin (Vg) of Frankliniella
occidentalis. [Methods] The
effect of single, and multiple, infections of L. attenuatum on Vg
expression and content in adult female F. occidentalis were studied from the F1 to the F3 generations. [Results] Expression
of Vg in the F2 generation
was significantly higher than in the control when second instar larvae produced
by gamogenesis were only infected once by L. attenuatum (P<0.05). Vg expression
was markedly higher in the F1 generation compared to the
control when second instar larvae produced by gamogenesis were repeatedly
infected by L. attenuatum (P<0.05). Vg expression levels in F2 and F3 generations produced by parthenogenesis were significantly
higher than in the control following both single and multiple infections (P<0.05). Vg content of F1 and F2 generations produced by gamogenesis, were markedly higher
than that of the control (P<0.05).
Vg content of F1 and F2 generations produced by
parthenogenesis were markedly higher than that of the control after a single
infection by L. attenuatum (P<0.05),
however, the Vg content of all three generations increased significantly after
multiple infections (P<0.05). [Conclusion] Infection with L. attenuatum significantly affects Vg expression and content in F. occidentalis, with the effect varying with the
frequency of infection, the generation of F. occidentalis, and the mode of
reproduction of F. occidentalis (gamogenesis or
parthenogenesis).