草地贪夜蛾卵和不同龄期幼虫对虱螨脲的敏感性及其与酶活力的关系
The susceptibility of eggs and different larval instars of the fall armyworm (Spodoptera frugiperda) to lufenuron, and the correlation between susceptibility to this pesticide and enzyme activity
王芹芹** 郏泊海 施 艳 何磊鸣 汪梅子 赵 特 周 琳***
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DOI：10.7679/j.issn.2095-1353.2026.013
作者单位：河南农业大学植物保护学院，小麦玉米两熟高效生产全国重点实验室，河南省新型农药创制与应用重点实验室， 河南省绿色农药创制工程技术研究中心，郑州 450046
中文关键词：草地贪夜蛾；虱螨脲；生物测定；解毒酶；几丁质酶
英文关键词： Spodoptera frugiperda; lufenuron; bioassays; detoxifying enzyme; chitinase
中文摘要：
【目的】 明确草地贪夜蛾Spodoptera frugiperda卵和不同龄期幼虫对虱螨脲的敏感性及其与解毒酶[多功能氧化酶（Mixed function oxidase，MFO）、羧酸酯酶（Carboxylesterase，CarEs）和谷胱甘肽S-转移酶（Glutathione S-transferase，GSTs）]和几丁质酶（Chitinase，CH）活性的相关性。【方法】 采用室内生物测定方法检测草地贪夜蛾卵和不同龄期幼虫对虱螨脲的敏感性，并检测了卵和不同龄期幼虫体内MFO、CarEs、GSTs以及CH的活力。【结果】 草地贪夜蛾卵和不同龄期幼虫对虱螨脲的敏感性呈先逐渐上升后迅速下降的趋势。其中，卵、5龄和6龄幼虫对虱螨脲的敏感性最低，LC50介于107.67-1 003.81 μg/mL。但1龄、2龄、3龄和4龄幼虫对虱螨脲的LC50分别为11.90、10.77、1.38 和2.26 μg/mL，相对毒力指数分别为84.35、93.20、727.40和444.16。经线性拟合分析发现，1-4龄幼虫对虱螨脲的相对毒力指数与MFO和GSTs酶比活力呈负相关，而与CH活性呈极显著正相关（P＜0.01）。【结论】 草地贪夜蛾卵和不同龄期幼虫对虱螨脲的敏感性存在显著差异，且1-4龄幼虫体内的CH活性增强可能有利于虱螨脲发挥毒力。虱螨脲可作为防治高龄草地贪夜蛾幼虫的理想药剂。
英文摘要：
[Aim] To
determine the susceptibility of eggs and different larval instars of the fall
armyworm (Spodoptera frugiperda) to lufenuron, and
the correlation between susceptibility to this pesticide and the activity of
detoxifying enzymes [mixed-function oxidase (MFO), carboxylesterase (CarEs),
glutathione S-transferase (GSTs)], and chitinase (CH)]. [Methods] Bioassays
were conducted and the activity of MFO, CarEs, GSTs, and CH in eggs and
different larval instars of S. frugiperda was measured in the laboratory. [Results] The susceptibility of eggs
and different larval instars to lufenuron gradually increased, then rapidly
decreased. Eggs and 5th and 6th instar larvae were the least sensitive to
lufenuron, with LC50 values between 107.67 and 1 003.81 μg/mL. In contrast, the LC50 values of 1st to 4th instar larvae were 11.90, 10.77, 1.38, and 2.26 μg/mL,
respectively, and the corresponding toxicity indices were 84.35, 93.20, 727.40,
and 444.16, respectively. Linear regression indicates that the relative
toxicity indices of 1st to 4th instar larvae were negatively correlated with
MFO and GSTs enzyme activity, but positively correlated with CH activity (P <0.01). [Conclusion] The
susceptibility of eggs and different larval instar of S. frugiperda to lufenuron were found to be significantly
different. Increased CH activity in 1st- to 4th-instar larvae may exacerbate
the toxicity of lufenuron. On the basis of these results, lufenuron is an ideal
insecticide for controlling higher instar S.
frugiperda larvae.