【目的】 探究浙江省内中华蜜蜂Apis cerana cerana（简称中蜂）的常见疾病流行情况，为蜂农和学者实施疾病预防并改进蜜蜂健康管理提供帮助。【方法】 于2023年7-12月采集浙江省内11个饲养点的74个中华蜜蜂工蜂蜂群，采用PCR及RT-PCR方法检测10种蜜蜂常见疾病病原在蜂群内的发病比例，分别是7种病毒病原：急性麻痹病毒（Acute bee paralysis virus，ABPV）、黑蜂王台病毒（Black queen cell virus，BQCV）、慢性麻痹病毒（Chronic bee paralysis virus，CBPV）、残翅病毒（Deformed wing virus，DWV）、以色列急性麻痹病毒（Israeli acute paralysis virus，IAPV）、克什米尔病毒（Kashmir bee virus，KBV）、中蜂囊状幼虫病毒（Chinese Sacbrood virus，CSBV）；2种细菌病原：欧洲幼虫腐臭病（European foulbrood，EFB）的病原蜂房球菌Melissococcus plutonius、美洲幼虫腐臭病（American foulbrood，AFB）的病原幼虫芽孢杆菌Paenibacillus larvae以及1种寄生虫病原：微孢子虫（Nosema spp.，NOS）。【结果】 在中蜂样品中有DWV、IAPV、KBV、CSBV、NOS和EFB这6种病原存在，其中EFB、NOS、CSBV和DWV的病原检出率较高，EFB的病原在11个市（县）中蜂中检出，且有8个市（县）的检出率超过50%，甚至有3个市（县）检出率达100%，说明其感染能力很强。NOS在8个市（县）中蜂中均有检出，且有6个市（县）的检出率超过50%；CSBV在3个市（县）中蜂中检出，且有2个市（县）的感染率超过60%；有3个市（县）中蜂检测出DWV阳性，且有1个市（县）感染率达100%；IAPV和KBV偶有检出。并且出现多种混合感染模式（52.71%），其中双重感染模式EFB与NOS的混合感染最多，达到35.14%，其次是CSBV与EFB的混合感染（5.41%），此外还检测出三重感染的情况（5.41%）。【结论】 浙江省中华蜜蜂感染EFB与NOS疾病的比例较高，同时存在一个蜂群感染2-3种病原的情况，说明浙江省中蜂蜂群存在一定的生存压力。调查结果对合理定制浙江省中蜂病害防治工作具有重要意义。

 [Aim]  To explore the prevalence of common diseases in Apis cerana cerana in Zhejiang Province, and thereby provide important information for honey bee health management. [Methods]  Samples were collected from 74 A. cerana cerana worker bee colonies from 11 apiary sites in Zhejiang from July to December 2023 and PCR and RT-PCR used to determine the prevalence of 10 honeybee pathogens. These included seven viral pathogens: Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV), Chinese Sacbrood virus (CSBV). We also screened for two bacterial pathogens: Melissococcus plutonius, which causes European foulbrood (EFB) and Paenibacillus larvae, which causes American foulbrood (AFB), plus one parasite Nosema spp. (NOS). [Results]  DWV, IAPV, KBV, CSBV, NOS and EFB were detected, of which EFB, NOS, CSBV and DWV were particularly prevalent. EFB was detected in all 11 cities and counties, eight of which had more than 50% infection rates, including three with 100% positive samples. Nosema spp. was detected in 8 counties, 6 of which had more than 50% prevalence. CSBV was identified in three counties, two with more than 60% infection rates. DWV positivity was observed in three counties, one of which had a 100% infection rate. IAPV and KBV were sporadically detected, while ABPV, CBPV, BQCV and AFB were absent. Mixed infections were prevalent (52.71%), with EFB-NOS co-infection being most common (35.14%), followed by CSBV-EFB (5.41%). Triple infections accounted for 5.41% of cases. [Conclusion]  The high prevalence of EFB and NOS infections in A. cerana cerana colonies from Zhejiang Province indicates a significant pathogen load on local bee populations. The co-existence of dual or triple pathogen infections further underscores this finding. These results provide essential information for formulating region-specific disease management strategies.