【目的】 褐飞虱Nilaparvata lugens是水稻的重要害虫，对水稻的生产造成严重破坏。本文旨在明确褐飞虱NlAKT基因对褐飞虱取食和繁殖的影响。【方法】 基于褐飞虱基因组获得NlAKT的全长开放阅读框（Open reading frame, ORF）序列并通过软件分析NlAKT的蛋白序列；利用RT-qPCR 技术分析其时空表达模式，最后通过RNA干扰（RNA interference, RNAi）的方法，分析NlAKT对NlVg和NlVgR基因表达水平的影响，研究NlAKT对褐飞虱取食、卵巢发育和卵巢内脂滴的影响。【结果】 分析NlAKT的蛋白序列发现NlAKT具有Akt蛋白的特征，有2个保守结构域（分别为 PH_PKB、STKc_PKB）。RT-qPCR结果表明NlAKT在各个发育阶段均有表达，在卵巢组织和成虫期高表达。对NlAKT进行RNAi后，NlAKT的表达降低了88%（P<0.05）；沉默 NlAKT后褐飞虱的蜜露量和获得性体重分别降低了51.6%和146.0%（P<0.05），取食孔数量增加了56.4%（P<0.05），这些指标与对照相比均有显著差异；此外沉默NlAKT后NlVg和NlVgR基因的表达水平分别降低了41.4%和59.3%（P<0.05），使卵巢发育延迟，卵巢内脂滴的大小和分布异常。【结论】 NlAKT在褐飞虱各发育阶段和组织均有表达，沉默NlAKT基因的表达后降低褐飞虱的取食，抑制卵巢的发育，并影响卵巢中脂滴的大小。这些结果表明NlAKT可调控褐飞虱的取食和繁殖。

 [Aim]  To clarify the role of the NlAKT gene in the feeding and reproduction of the brown planthopper (BPH), Nilaparvata lugens, an important insect pest of rice. [Methods]  The full-length open reading frame (ORF) sequence of NlAKT was obtained based on the BPH genome, and its protein sequence was analyzed using bioinformatics software. RT-qPCR technology was used to investigate its spatiotemporal expression pattern. Finally, RNA interference (RNAi) methodology was used to determine the effect of NlAKT on the expression of the NlVg and NlVgR genes, along with its effects on feeding, ovarian development, and lipid droplet accumulation in the ovaries. [Results]  The NlAKT protein sequence contains characteristic Akt protein domains, including two conserved domains (PH_PKB and STKc_PKB). RT-qPCR results indicate that NlAKT is expressed in all tissues and developmental stages, with higher expression in ovarian tissues and during the adult stage. RNAi significantly reduced NlAKT expression by 88% (P < 0.05). Silencing NlAKT led to a 51.6% decrease in honeydew excretion, a 146% reduction in acquired body weight (P < 0.05), and a 56.4% increase in the number of feeding pores (P < 0.05). All the above parameters were significantly different from those of the control group. Furthermore, knockdown of NlAKT reduced the expression of the NlVg and NlVgR genes by 41.4% and 59.3%, respectively (P < 0.05), resulting in delayed ovarian development and abnormal size and distribution of lipid droplets within the ovaries. [Conclusion] NlAKT is expressed across all developmental stages and tissues of the brown planthopper. Silencing the NlAKT gene reduced feeding behavior, inhibited ovarian development, and affecting lipid droplet size in the ovaries. These results indicate that NlAKT regulates feeding and reproduction in the BPH.