【目的】 本研究以番茄潜叶蛾Tuta absoluta幼虫为对象，系统探究温度双向胁迫对其抗氧化与解毒酶活性的影响及其动态响应机制。【方法】 通过模拟不同温度梯度（﹣10、﹣5、0、26、35、37.5和40 ℃）及处理时长（0.5、1和2 h），测定了超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化物酶（Peroxidase，POD）、过氧化氢酶（Catalase，CAT）、总抗氧化能力（Total antioxidant capacity，T-AOC）、谷胱甘肽S-转移酶（Glutathione S-transferase，GST）、多功能氧化酶（Multifunctional oxidase，MFO）及羧酸酯酶（Carboxylesterase，CarE）的活性变化，并结合相关性分析揭示了其协同调控规律。【结果】 极端低温短时处理显著激活SOD、POD和CAT活性，但长时暴露导致活性显著衰减（P<0.05）；而高温初期抑制CAT和MFO活性，但2 h后GST活性爆发性升高，表明温度脉冲对酶活性的调控具有显著时效差异（P<0.05）。SOD、POD、CAT与T-AOC呈强正相关（P<0.05），形成链式抗氧化网络；GST与MFO显著负相关（P<0.05），高温下通过代谢分流维持解毒功能。37.5 ℃为功能失衡临界温度，其诱导T-AOC显著升高但抑制MFO活性（P<0.05）；而0 ℃短时处理特异性激活CarE，提示其对寄主植物酯类毒素的适应性响应。【结论】 本研究首次揭示了番茄潜叶蛾幼虫在温度双向胁迫下的“急性代偿-代谢分流-临界阈值”动态响应规律，为全球外来入侵有害生物的综合防控技术研发提供了理论依据。

 [Aim]  This study aimed to systematically investigate the effects of bidirectional temperature stress on the antioxidant and detoxification enzyme activities of Tuta absoluta larvae and their dynamic response mechanisms. [Methods]  Various temperature gradients (-10, -5, 0, 26, 35, 37.5, 40 °C) and exposure durations (0.5, 1, 2 h) were simulated to measure the activity changes of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), total antioxidant capacity (T-AOC), glutathione S-transferase (GST), multifunctional oxidase (MFO), and carboxylesterase (CarE). Correlation analysis was performed to reveal their coordinated regulatory mechanisms. [Results]  Short-term exposure to extreme low temperatures significantly activated SOD, POD, and CAT activities, whereas prolonged exposure led to a marked decline. In contrast, high temperatures initially inhibited CAT and MFO activities, but GST activity surged after 2 hours, indicating that temperature fluctuations have distinct time-dependent regulatory effects on enzyme activity. SOD, POD, and CAT exhibited a strong positive correlation with T-AOC, forming a chain-like antioxidant network. GST showed a significant negative correlation with MFO, maintaining detoxification function via metabolic shunting under high-temperature conditions. The critical threshold temperature for functional imbalance was identified as 37.5 °C, which significantly increased T-AOC but suppressed MFO activity. Moreover, short-term exposure to 0 °C specifically activated CarE, suggesting an adaptive response to host plant ester toxins. [Conclusion]  This study is the first to reveal the "acute compensation-metabolic shunting-critical threshold" dynamic response pattern of T. absoluta larvae under bidirectional temperature stress, providing a theoretical foundation for the integrated management of invasive pest species worldwide.