【目的】 花蓟马Frankliniella intonsa是为害黄花菜的重要害虫，本研究旨在探究多杀菌素类农药对花蓟马的毒力、发育繁殖、保护酶和解毒酶活性的影响。【方法】 分别采用饲喂法和浸虫法测定乙基多杀菌素（Spinetoram）和多杀菌素（Spinosad）对花蓟马成虫、若虫和蛹的毒力，以及亚致死浓度（LC₂₅）处理对花蓟马发育历期和繁殖力的影响，并探究LC₂₅胁迫6、12、18和24 h花蓟马体内保护酶超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化氢酶（Catalase，CAT）、过氧化物酶（Peroxidase，POD）和解毒酶谷胱甘肽S-转移酶（Glutathione S-transferase，GSTs）、羧酸酯酶（Caboxylesterase，CarE）活性的变化趋势。【结果】 花蓟马不同发育阶段（雌成虫、2龄若虫、蛹）对乙基多杀菌素均表现出显著敏感性，其半致死浓度LC₅₀依次为0.004 4、0.004 7和0.023 4 mg/L。两种多杀菌素类农药LC₂₅胁迫下，花蓟马的产卵前期较对照均显著延长（P<0.05），同时，1龄若虫期均极显著延长（P<0.001）。多杀菌素LC₂₅胁迫下，花蓟马体内保护酶活性变化较为显著，除6 h的POD活性和18 h的SOD活性，胁迫24 h内，3 种保护酶SOD、CAT和POD活性在6、12、18和24 h均极显著升高（P<0.01）；解毒酶GSTs活性在24 h内显著升高（P<0.05）。乙基多杀菌素LC₂₅胁迫下，花蓟马CAT活性在6、12、18和24 h相比对照均极显著升高（P<0.01），SOD和POD活性变化相对平稳，胁迫24 h后二者同对照无显著差异（P>0.05），解毒酶GSTs和CarE在24 h内均显著高于对照（P<0.05）。【结论】 乙基多杀菌素对花蓟马不同虫态的毒力效果显著；LC₂₅多杀菌素类农药胁迫主要影响花蓟马的产卵前期和1龄若虫期；多杀菌素能够诱导花蓟马SOD、CAT和POD协同响应保护虫体，激活GSTs代谢解毒；乙基多杀菌素胁迫对花蓟马保护酶影响不明显，主要诱导CAT活性升高，解毒酶GSTs和CarE活性均被激活。

 [Aim]  To investigate the toxicity of the pesticides spinetoram and spinosad to Frankliniella intonsa, an important pest of daylilies, and on the activity of protective and detoxifying enzymes in this species. [Methods]  The feeding and immersion methods were used to determine the toxicity of spinetoram and spinosad to adults, nymphs, and pupae of F. intonsa. The effects of a sublethal concentration (LC₂₅) of spinetoram and spinosad on the developmental duration and fecundity of F. intonsa were measured using the feeding method. In addition, changes in the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione S-transferases (GSTs) and carboxylesterase (CarE), were measured 6, 12, 18, and 24 hours, after exposure to LC₂₅ doses of spinetoram and spinosad. [Results]  Different developmental stages (female adults, 2nd instar nymphs and pupae) of F. intonsa showed significant susceptibility to spinetoram, with corresponding LC₅₀ values of 0.004 4 mg/L, 0.004 7 mg/L, and 0.023 4 mg/L, respectively. Exposure to LC₂₅ levels of both spinetoram and spinosad, significantly prolonged the pre-oviposition period (P < 0.05) and the duration of the 1st-instar nymph stage (P < 0.001) relative to the control. Protective enzyme activity changed markedly after exposure to an LC₂₅ doses of spinosad. With the exception of POD at 6 h and SOD at 18 h, activity of SOD, CAT, and POD increased significantly at 6, 12, 18, and 24 h post-exposure (P < 0.01), relative to the control. GSTs activity also increased significantly over 24 h (P < 0.05). CAT activity significantly increased relative to the control at 6, 12, 18, and 24 h after exposure to an LC₂₅ dose of spinetoram  (P<0.01), whereas SOD and POD activity was not significantly different to that of the control after 24 h (P>0.05). GSTs and CarE activity significantly increased compared to the control over 24 h (P<0.05). [Conclusion]  An LC₂₅ dose of spinetoram is toxic to different developmental stages of F. intonsa. The duration of the pre-oviposition period and 1st-instar nymph stage were significantly prolonged by exposure to LC₂₅ doses of spinetoram and spinosad. Spinosad induces a synergistic increase in SOD, CAT, and POD activity, and activates GSTs for metabolic detoxification. Spinetoram had no significant effect on protective enzymes but induced an increase in CAT activity. Both detoxifying GSTs and CarE were activated by exposure to this pesticide.