【目的】 分离鉴定造成白星花金龟Protaetia brevitarsis死亡的病原菌，研究防治手段，为有效利用资源昆虫白星花金龟提供理论依据。【方法】 平板培养法分离纯化白星花金龟病虫虫体上的病原菌，采用生物测定法，测定分离菌株的致病力，并依据形态学和分子生物学鉴定手段对分离得到的病原菌进行物种鉴定。将分离到的菌株接种到白星花金龟饲养基料中，选取7种化学农药进行药剂筛选试验，设置不接菌+药剂、接菌+药剂、接菌发病后+药剂3组不同处理，调查幼虫死亡率和体重增长率。【结果】 经分离鉴定，该菌为金龟子绿僵菌Metarhizium anisopliae。人工接种病原菌14 d后白星花金龟1龄、2龄、3龄幼虫的死亡率分别为100.00%、91.67%、76.00%。药剂筛选试验结果显示20%三唑酮乳油效果最好，死亡率仅为12.22%±2.22%，较对照降低了62%的死亡率。【结论】 在养虫基料中添加20%三唑酮乳油可有效预防幼虫发病，幼虫发病后再添加药剂效果较差。本研究为白星花金龟人工繁殖提供了理论研究数据和技术支持。

 [Aim]  Isolate and identify the fungus pathogenic that cause the death of Protaetia brevitarsis, and develop methods to reduce mortality during the artificial rearing of this species. [Methods]  A pathogenic fungal strain was isolated and purified using the plate culture method. Bioassays were used to determine the pathogenicity of the isolated strain, which was identified using morphological and molecular biological identification methods. The isolated strain was inoculated into feed provided to P. brevitarsis, and the effectiveness of 7 chemical fungicidesat suppressing fungal growth were evaluated. Three different treatments were set up: No fungi + fungicide, fungi + fungicide, and fungal infection + fungicide. The mortality and growth rate of the larvae under each treatment were measured and compared. [Results]  The pathogenic fungal strain was identified as Metarhizium anisopliae. Mortality rate of the 1st, 2nd and 3rd instars was 100.00%, 91.67% and 76.00%, respectively, 14 days after inoculation. The most effective treatment was 20% triadimefonemulsifiable concentrate, which reduced mortality tojust 12.22%±2.22%; a 62% reduction compared to the control. [Conclusion]  Adding 20% triadimefonemulsifiable concentrate to the diet of captive P. brevitarsis effectively preventedfungal infection, but was not very effective at reducing mortality onceinfection had occurred.