【目的】 细胞色素P450酶基因在植食性昆虫代谢寄主植物次生物质中发挥重要的作用，研究CYP450响应植物次生物质的转录水平对于害虫的防治具有指导意义。本研究旨在探究单宁酸对豌豆蚜Acyrthosiphon pisum细胞色素P450s酶活性及CYP4和CYP6家族基因表达的影响。【方法】 分别用0.5、1和2 g·L^﹣1单宁酸处理3龄和4龄豌豆蚜48 h，测定其体内CYP450s酶活性，并分析豌豆蚜CYP4和CYP6家族基因在不同组织响应单宁酸的表达模式。【结果】 以取食清水处理后蚕豆叶片的豌豆蚜为对照。2.0 g·L^﹣1单宁酸处理可诱导2个龄期豌豆蚜CYP450酶活性显著升高（P<0.05）。取食单宁酸48 h后，头部有11个基因表达显著上调（P<0.05），其中ApCYP4C1014、ApCYP4C1019和ApCYP6A1401表达量分别为对照的3.13、3.02和3.93倍；腹部有14个基因显著上调，包括ApCYP4C109、ApCYP4C1011等9个基因与对照相比上调超5倍（P<0.05）；中肠ApCYP4C1021和ApCYP6A1305与对照相比分别上调2.90和2.03倍（P<0.05）。【结论】 CYP4和CYP6家族基因可能参与豌豆蚜对单宁酸的代谢。

 [Aim]  To investigate the effect of tannic acid on cytochrome P450s enzyme activity and related gene expression in the pea aphid Acyrthosiphon pisum. [Methods]  3rd and 4th instar pea aphids were treated with tannic acid at doses of 0.5, 1 or 2 g·L^﹣1 for 48 h, respectively. [Results]  Pea aphids fed on broad bean leaves treated with clean water served as the control. The 2.0 g·L^﹣1 tannic acid treatment induced a significant increase in CYP450 enzyme activity in both instars (P < 0.05). Eleven genes in the head were significantly up-regulated after 48 h of tannic acid treatment (P < 0.05), among these the expression levels of ApCYP4C1014, ApCYP4C1019 and ApCYP6A1401 were 3.13-fold, 3.02-fold, and 3.93-fold higher than those of the control, respectively. Fourteen genes were significantly up-regulated in the abdomen, including nine, such as ApCYP4C109 and ApCYP4C1011, which were up-regulated more than 5-fold (P < 0.05). ApCYP4C1021 and ApCYP6A1305 were up-regulated in the mid-gut 2.90-fold and 2.03-fold, respectively (P < 0.05). [Conclusion]  The results suggest that CYP4 and CYP6 family genes may be involved in the metabolism of tannic acid by the pea aphid.