雷氏萤Aquatica leii热休克蛋白超基因家族的全基因组鉴定及其在高温胁迫应答中的表达
Genome-wide identification of the Aquatica leii heat shock protein and analysis of their expressions in response to high-temperature stress
余航远1** 汪法能1 江 晓1 李佳鹏2 曹成全3 王义平2***
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DOI：10.7679/j.issn.2095-1353.2026.076
作者单位：1. 钱江源国家公园管理局，衢州 324300；2. 浙江农林大学，林业与生物技术学院，杭州 311300；3. 乐山师范学院，生命科学学院，乐山 614004
中文关键词：雷氏萤；生物信息学；热休克蛋白；高温胁迫
英文关键词： Aquatica leii; bioinformatics; heat shock protein; high-temperature stress
中文摘要：【目的】 热休克蛋白（Heat shock protein, Hsp）在动植物生长发育和胁迫响应中有着重要的作用。然而，关于雷氏萤Aquatica leii Hsp超基因家族的鉴定和功能特征的信息较少。本研究旨在鉴定雷氏萤中Hsp基因家族及其在高温响应中的表达。【方法】 使用生物信息学鉴定雷氏萤Hsp超基因家族成员，并通过转录组分析了Hsp在高温胁迫响应中的表达情况。【结果】 Hsp家族包含45个Hsp成员，分别是3个Hsp90、19个Hsp70、9个Hsp60和14个sHsp基因。在雷氏萤蛹不同发育阶段的发光器中，大部分Hsp的表达呈现出逐渐降低的趋势。高温胁迫下幼虫转录组分析表明，AlHsp70-7、AlHsp70-8、AlsHsp17.44、AlsHsp17.49、AlsHsp20.77和AlsHsp29.38被温度胁迫所诱导，随着温度的增加，表达量也在逐步增加。【结论】 在28和32 ℃胁迫下分别有12和13个Hsp基因显著上调，其在响应高温胁迫应答中可能发挥着重要的作用。这些结果为进一步探索其功能奠定了良好的基础。
英文摘要：[Aim] To identify the Hsp gene family of Aquatica leii and quantify its expression in response to high temperatures. [Methods] The Hsp supergene family of A. leii was identified using bioinformatics, and its expression in response to high temperature stress was analyzed using transcriptome analysis. [Results] The AlHsp family contains 45 Hsp family members, including 3 Hsp90, 19 Hsp70, 9 Hsp60 and 14 sHsp genes. The number of Hsp genes expressed gradually decreased in the luminescent organs of different developmental stages. Transcriptome analysis of larvae under high temperature stress showed that AlHsp70-7, AlHsp70-8, AlsHsp17.44, AlsHsp17.49, AlsHsp20.77 and AlsHsp29.38, were induced by temperature stress, and that expression progressively increased with increasing temperature. [Conclusion] At 28 and 32 ℃, the 12 and 13 Hsp genes were significantly upregulated, which suggests that these play an important role in the response to high temperature stress. These results lay a solid foundation for further investigation of the function of the Hsp gene family.