唑虫酰胺和溴氰菊酯胁迫下桑螟肠道代谢物的变化
Effects of tolfenpyrad and deltamethrin on the intestinal metabolome of Glyphodes pyloalis
梁 静1, 2** 容万韬2 薛胜昊2 梁恩希2 曾慧玲2 武正军1*** 李晓东1, 2***
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DOI：10.7679/j.issn.2095-1353.2026.085
作者单位：1. 广西师范大学生命科学学院，珍稀濒危动植物生态与环境保护教育部重点实验室，桂林 541006；2. 河池学院化学与生物工程学院，广西蚕桑生态学与智能化技术应用重点实验室，河池 546300
中文关键词：桑螟；唑虫酰胺；溴氰菊酯；农药；代谢组学；代谢通路
英文关键词：Glyphodes pyloalis; tolfenpyrad; deltamethrin; pesticide; metabolomics; metabolic pathways
中文摘要：【目的】 探究唑虫酰胺和溴氰菊酯两种常用杀虫剂胁迫对桑螟Glyphodes pyloalis 肠道代谢物的影响。【方法】 设置未进行农药处理对照组（DD），唑虫酰胺处理组（DC）和溴氰菊酯处理组（DX）共3组桑螟肠道样品，利用超高效液相色谱-串联静电场轨道阱质谱联用仪（UHPLC-Q Exactive HFX，Thermo，USA）分析其肠道代谢物。通过Progenesis QI软件处理数据，采用主成分分析（PCA）、偏最小二乘法判别分析（PLS-DA）及正交偏最小二乘法判别分析（OPLS-DA）筛选差异代谢物（筛选标准：P<0.05，VIP>1，FC>1.5或FC<0.667），并进行KEGG代谢通路富集分析。【结果】 不同处理组桑螟肠道代谢物存在差异，与DD组相比，DC组和DX组分别鉴定出74和70个差异代谢物，两组共有差异代谢物35个（5个上调，30个下调）。差异代谢物主要归属于甘油磷脂、嘧啶核苷、有机含氧化合物、羧酸及其衍生物。通路富集分析表明，DC组和DX组差异代谢物主要富集于碳水化合物代谢、氨基酸代谢和核苷酸代谢等通路。【结论】 唑虫酰胺与溴氰菊酯胁迫可特异性地扰动桑螟肠道代谢谱，并主要干扰氨基酸、碳水化合物等相关代谢通路，这些特征性代谢变化可能是桑螟应对农药胁迫的重要生理响应。
英文摘要：[Aim] To investigate the effects of two commonly used insecticides, tolfenpyrad and deltamethrin, on the intestinal metabolites of the Glyphodes pyloalis. [Methods] A total of three G. pyloalis treatment groups were established; a non-pesticide treatment control group (DD), a tolfenpyrad treatment group (DC) and a deltamethrin treatment group (DX). Intestinal metabolites were analyzed by ultra-high performance liquid chromatography-tandem, electrostatic, field orbitrap, mass spectrometry (UHPLC-Q Exactive HFX, Thermo, USA). Data were processed using progenesis QI software. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA), were used to screen differential metabolites (screening criteria: P<0.05, FC > 1.5 or < 0.667). KEGG metabolic pathway enrichment analysis was also conducted. [Results] There were significant differences in the intestinal metabolites of G. pyloalis among the different treatment groups. Compared to the DD group, 74 and 70 significantly differential metabolites were identified in the DC and DX groups, respectively, with 35 common, differential metabolites shared between these groups (5 upregulated and 30 downregulated). The differential metabolites were mainly attributed to glycerophospholipids, pyrimidine nucleosides, organic oxygenates, carboxylic acids and their derivatives. Pathway enrichment analysis showed that differential metabolites in the DC and DX groups were mainly enriched in the carbohydrate, amino acid and nucleotide, metabolisms. [Conclusion] Tolfenpyrad and deltamethrin can specifically disturb the intestinal metabolic spectrum of G. pyloalis, mainly by interfering with related metabolic pathways, such as amino acids and carbohydrates. These characteristic metabolic changes may be important physiological responses of G. pyloalis to pesticide stress.